Search tool

Use this search to find references that are related to a specific probe/primer and/or that contain a given keyword. The search options can be used to limit the number of probes that are shown in the results, by only showing probes that have been used for in situ hybridization (FISH), are part of microarrays, or showing only primers.

The specificity entry of each probe/primer indicates the intended target organism(s) as described in the original publication or during user submissions to probeBase. Furthermore, each oligonucleotide in probeBase is assigned to a taxon in the NCBI taxonomy. Please note that this taxonomic assignment does not necessarily mean that an oligonucleotide is highly specific for a given taxon, but it allows for more advanced searches. A search for a target organism or taxon queries the specificity and taxonomy entries and systematically returns all available oligonucleotides. The search can be further adjusted by including oligonucleotides that target higher and/or lower taxonomic ranks than the query taxon. The output list of probes that target the query organism at different taxonomic levels helps researchers to identify sets of hierarchically nested probes for application in multiple-probe hybridization formats such as multi-color FISH and DNA microarrays.
The search options can be used to limit the number of probes that are shown in the results, by only showing probes that have been used for in situ hybridization (FISH), are part of microarrays, or showing only primers.

Entering high taxonomic levels with this option selected may slow down the search.
The highest taxonomic level for this option is domain (Bacteria, Eukaryota, Archaea). To include all domains, use the lists tool instead.

Use this search function to identify probes/primers that bind in a defined region of the rRNA gene by entering the binding position according to the E. coli numbering (e.g. enter 338 to find all oligonucleotides that bind near the target position of probe EUB338). This tool further assists in the development of new oligonucleotide probes for FISH by providing information on whether a searched target site has previously been found accessible for oligonucleotide probes in other microorganisms.
The search options can be used to limit the number of probes that are shown in the results, by only showing probes that have been used for in situ hybridization (FISH), are part of microarrays, or showing only primers.

Probes with start or end position at the entered position ± 20 nt will be shown.

Use this search to find oligonucleotides with a specific sequence. Up to 2 mismatches can be allowed. Amibguous characters will be translated according to IUPAC code.
The search options can be used to limit the number of probes that are shown in the results, by only showing probes that have been used for in situ hybridization (FISH), are part of microarrays, or showing only primers.

At least 6 characters must be entered.
Only enter valid characters for DNA/RNA sequences. Allowed characters: ACGTURYSWKMBDHVNXIacgturyswkmbdhvnxi-.