Details of the microarray

Array name Description Technique Reference
Bacillus-PhyloChip Low-density oligonucleotide microarray (microchip) for identifying strains of five closely related bacilli (Bacillus anthracis, Bacillus cereus, Bacillus mycoides, Bacillus medusa and Bacillus subtilis). The microarray contains a hierarchical set of 30 oligonucleotide probes targeting the 16S ribosomal RNA of these bacilli at multiple levels of specificity (approximate taxonomic ranks of domain, kingdom, order, genus and species). Low-density DNA microchip array, consisting of oligonucleotide probes embedded in glass-immobilized polyacrylamide gel elements measuring 100x100x20µm and spaced 200µm apart on a glass slide, produced by a photopolymerization procedure. Optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approach. Liu WT, Mirzabekov AD, Stahl DA. Environmental microbiology. 2001. Pubmed: 11722542

The following probes are part of the array:

EUB338 (Bact338) (bacterial) (pB-159), 10 (pB-385), 11 (pB-386), 12 (pB-387), 13 (pB-388), 14 (pB-389), 15 (pB-390), 16 (pB-391), 17 (pB-392), 18 (pB-393), 19 (pB-394), 20 (pB-395), 21 (pB-396), 22 (pB-397), 23 (pB-398), 24 (pB-399), 25 (pB-400), 26 (pB-401), 27 (pB-402), 28 (pB-403), 29 (pB-404), 30 (pB-405), 3 (pB-542), 6 (pB-543), 7 (pB-544), 8 (pB-545), 9 (pB-546), 1 (pB-549), 2 (pB-550), 5 (pB-551)