Probe details

MET1217

Tested for in situ hybridization.

Accession no. pB-916
Full name (Alm et al.) -
Categories methylotrophic bacteria
Specificity Methylophilus
Taxonomy Methylophilus; Methylophilaceae; Nitrosomonadales; Betaproteobacteria; Pseudomonadota; Pseudomonadati; Bacteria; cellular organisms
Sequence 5'-TTA CGT GTG AAG CCC TGG-3'
Length [nt] 18
G+C content [%] 56
Target rRNA 16S
Position 1217-1234
Formamide 20
Check specificity/coverage SILVA TestProbe
References Microbial community and physicochemical analysis of an industrial waste gas biofilter and design of 16S rRNA-targeting oligonucleotide probes. Friedrich U, Van Langenhove H, Altendorf K, Lipski A. Environmental microbiology. 2003. Pubmed: 12588298
Remarks molar ratio of competitor oligonucleotide versus probe is 1 helper oligonucleotides: H979, CAAGGCCAGGTAAGG, H1199, CCATAAGGGCCAT GAGGA, H1235, GTACCGACCATWGTA