Probe details

A519R

To be used as primer.

Accession no. pB-3757
Full name (Alm et al.) S-D-Arch-0519-a-A-19
Categories primer
Specificity Archaea
Taxonomy Archaea; cellular organisms
Sequence 5'-GGT DTT ACC GCG GCK GCT G-3'
Length [nt] 19
G+C content [%] 63
Target rRNA 16S
Position 519-539
Direction Reverse primer
Tm 57.50
Check specificity/coverage SILVA TestProbe
References Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies. Wang Y, Qian PY. PloS one. 2009. Pubmed: 19816594
Remarks -

Coverage

Mismatches Archaea Bacteria Eukaryota
0 91.3419 0.1226 0.9722
1 95.9914 92.1062 89.3722