To be used as primer.
Accession no. | pB-3753 |
Full name (Alm et al.) | S-*-Univ-0519-a-S-18 |
Categories |
primer |
Specificity | Bacteria, Archaea, Eukarya |
Taxonomy | cellular organisms |
Sequence | 5'-CAG CMG CCG CGG TAA TWC-3' |
Length [nt] | 18 |
G+C content [%] | 61 |
Target rRNA | 16S |
Position | 519-536 |
Direction | Forward primer |
Tm | 54.00 |
Check specificity/coverage |
SILVA TestProbe |
References |
Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies. Wang Y, Qian PY. PloS one. 2009. Pubmed: 19816594 |
Remarks | - |
Mismatches | Archaea | Bacteria | Eukaryota |
0 | 55.1841 | 87.5855 | 89.1559 |
1 | 95.7063 | 97.8417 | 96.8478 |