Probe details

U519F

To be used as primer.

Accession no. pB-3753
Full name (Alm et al.) S-*-Univ-0519-a-S-18
Categories primer
Specificity Bacteria, Archaea, Eukarya
Taxonomy cellular organisms
Sequence 5'-CAG CMG CCG CGG TAA TWC-3'
Length [nt] 18
G+C content [%] 61
Target rRNA 16S
Position 519-536
Direction Forward primer
Tm 54.00
Check specificity/coverage SILVA TestProbe
References Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies. Wang Y, Qian PY. PloS one. 2009. Pubmed: 19816594
Remarks -

Coverage

Mismatches Archaea Bacteria Eukaryota
0 55.1841 87.5855 89.1559
1 95.7063 97.8417 96.8478