Probe details

534R

To be used as primer.

Accession no. pB-3725
Full name (Alm et al.) S-D-Bact-0517-a-A-18
Categories primer
Specificity Bacteria
Taxonomy Bacteria; cellular organisms
Sequence 5'-ATT ACC GCG GCT GCT GGC-3'
Length [nt] 18
G+C content [%] 67
Target rRNA 16S
Position 517-534
Direction Reverse primer
Tm 55.00
Check specificity/coverage SILVA TestProbe
References Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome. Nossa CW, Oberdorf WE, Yang L, Aas JA, Paster BJ, Desantis TZ, Brodie EL, Malamud D, Poles MA, Pei Z. World journal of gastroenterology. 2010. Pubmed: 20806429
Remarks -

Coverage

Mismatches Archaea Bacteria Eukaryota
0 0.4843 87.7853 86.2078
1 30.8174 98.0512 93.6153