Probe details

MX825

Tested for in situ hybridization.

Accession no. pB-232
Full name (Alm et al.) -
Categories methanogenic archaea
Specificity Methanosaeta spp.
Taxonomy Methanothrix; Methanotrichaceae; Methanotrichales; Methanomicrobia; Stenosarchaea group; Methanobacteriota; Methanobacteriati; Archaea; cellular organisms
Sequence 5'-TCG CAC CGT GGC CGA CAC CTA GC-3'
Length [nt] 23
G+C content [%] 70
Target rRNA 16S
Position 825-847
Formamide 50
Check specificity/coverage SILVA TestProbe
References Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens. Raskin L, Stromley JM, Rittmann BE, Stahl DA. Applied and environmental microbiology. 1994. Pubmed: 7517128
An update and optimisation of oligonucleotide probes targeting methanogenic Archaea for use in fluorescence in situ hybridisation (FISH). Crocetti G, Murto M, Björnsson L. Journal of microbiological methods. 2006. Pubmed: 16126291
Remarks wrong sequence in reference (should be reverse complementary)