Probe details
MS821
Tested for in situ hybridization.
|
| Accession no. |
pB-229 |
| Full name (Alm et al.) |
S-G-Msar-0821-a-A-24 |
| Categories |
methanogenic archaea
|
| Specificity |
Methanosarcina frisius, Methanosarcina spp., Methanosarcina acidovorans, Methanosarcina thermophila, Methanosarcina barkeri, Methanoholophilus mahii, Methanophilus sp. |
| Taxonomy |
Methanosarcinaceae;
Methanosarcinales;
Methanomicrobia;
Stenosarchaea group;
Methanobacteriota;
Methanobacteriati;
Archaea;
cellular organisms
|
| Sequence |
5'-CGC CAT GCC TGA CAC CTA GCG AGC-3' |
| Length [nt] |
24 |
| G+C content [%] |
67 |
| Target rRNA |
16S |
| Position |
821-844 |
| Formamide |
40 |
| Check specificity/coverage |
SILVA TestProbe
|
| References |
Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens. Raskin L, Stromley JM, Rittmann BE, Stahl DA. Applied and environmental microbiology. 1994. Pubmed: 7517128
An update and optimisation of oligonucleotide probes targeting methanogenic Archaea for use in fluorescence in situ hybridisation (FISH). Crocetti G, Murto M, Björnsson L. Journal of microbiological methods. 2006. Pubmed: 16126291
|
| Remarks |
wrong sequence in reference (should be reverse complementary) |