Probe details
MS1414
Tested for in situ hybridization.
|
| Accession no. |
pB-226 |
| Full name (Alm et al.) |
- |
| Categories |
methanogenic archaea
|
| Specificity |
Methanosarcina spp., Methanococcoides spp., Methanolobus spp., Methanohalophilus spp. |
| Taxonomy |
Methanosarcinaceae;
Methanosarcinales;
Methanomicrobia;
Stenosarchaea group;
Methanobacteriota;
Methanobacteriati;
Archaea;
cellular organisms
|
| Sequence |
5'-CTC ACC CAT ACC TCA CTC GGG-3' |
| Length [nt] |
21 |
| G+C content [%] |
62 |
| Target rRNA |
16S |
| Position |
1414-1434 |
| Formamide |
50 |
| Check specificity/coverage |
SILVA TestProbe
|
| References |
Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens. Raskin L, Stromley JM, Rittmann BE, Stahl DA. Applied and environmental microbiology. 1994. Pubmed: 7517128
An update and optimisation of oligonucleotide probes targeting methanogenic Archaea for use in fluorescence in situ hybridisation (FISH). Crocetti G, Murto M, Björnsson L. Journal of microbiological methods. 2006. Pubmed: 16126291
|
| Remarks |
helper oligonucleotides: hMS1395, GGTTTGACGGGCGGTGTG, hMS1480, CGACTTAACCCCCCTTGC |