Probe details

WJ20646

Tested for in situ hybridization.

Accession no. pB-1209
Full name (Alm et al.) -
Categories extremophilic microbes
Specificity acidophilic gamma-Proteobacteria WJ2 and PK51 and some acid mine drainage clones
Taxonomy Gammaproteobacteria; Pseudomonadota; Pseudomonadati; Bacteria; cellular organisms
Sequence 5'-TAC CGT ACT CCA GCA AGC-3'
Length [nt] 18
G+C content [%] 56
Target rRNA 16S
Position 646-663
Formamide 40
Check specificity/coverage SILVA TestProbe
References Macroscopic streamer growths in acidic, metal-rich mine waters in north wales consist of novel and remarkably simple bacterial communities. Hallberg KB, Coupland K, Kimura S, Johnson DB. Applied and environmental microbiology. 2006. Pubmed: 16517651
Remarks Requires helper oligonucleotides, unlabelled and added in equal concentrations to that of the probe. They are WJ20646h1 = GGGAATTCCACCTTTCTC and ABl1002h2 = CAGTATCCACCGCCATT.