Probe details

Lmali

Tested for in situ hybridization.

Accession no. pB-1011
Full name (Alm et al.) -
Categories
Specificity Lactobacillus mali
Taxonomy Liquorilactobacillus mali; Liquorilactobacillus; Lactobacillaceae; Lactobacillales; Bacilli; Bacillota; Bacillati; Bacteria; cellular organisms
Sequence 5'-AAG CAT TCG RTG AAA GTT TTG-3'
Length [nt] 21
G+C content [%] 33
Target rRNA 16S
Position 70-90
Formamide -
Check specificity/coverage SILVA TestProbe
References Development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria. Blasco L, Ferrer S, Pardo I. FEMS microbiology letters. 2003. Pubmed: 12900029
Remarks cell treatment with 10 µl of 0.1 mg lysozyme per ml in 100 mM Tris-HCl and 50 mM EDTA (pH 8.0) for 20 min at 4°C or 5 min at room temperature