Probe/primer details
| CFX1223 | Tested for in situ hybridization. |
| Accession no. | pB-719 |
| Taxonomy | Chloroflexi; Bacteria |
| Specificity | phylum Chloroflexi (green nonsulfur bacteria) |
| Category | phototrophic bacteria higher taxonomic levels |
| Target rRNA | 16S rRNA |
| Position | 1223-1242 |
| Sequence | 5'- CCA TTG TAG CGT GTG TGT MG -3' |
| G+C content [%] | 50 |
| Length [nt] | 20 |
| Check specificity/coverage | 
|
| Formamide [%] | 35 |
| Hybridization efficiency |  |
| References | Filamentous Chloroflexi (green non-sulfur bacteria) are abundant in wastewater treatment processes with biological nutrient removal. Björnsson L, Hugenholtz P, Tyson GW, Blackall LL. Microbiology (Reading, England). 2002. Pubmed |
| Remarks | Use probes GNSB-941 and CFX1223 in equimolar mixture to detect all Chloroflexi |
Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.
