Probe/primer details
| Name | ECO-2 |
| Accession no. | pB-713 |
| Taxonomy | Escherichia coli; Escherichia; Enterobacteriaceae; Enterobacteriales; Gammaproteobacteria; Proteobacteria; Bacteria |
| Specificity | Escherichia coli ATCC 25922 GenBank no. (GI) 7110561 |
| Target rRNA | 16S rRNA |
| Sequence | 5'- GGA AGG GAG TAA AGT TAA TAC CTT TGC TCA TTG ACG TTA C -3' |
| G+C content [%] | 40 |
| Check specificity/coverage | 
|
| Hybridization efficiency |  |
| References | Design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples. Wang RF, Beggs ML, Robertson LH, Cerniglia CE. FEMS microbiology letters. 2002. Pubmed |
| Remarks | See Table 1 in reference for orientation (5apos to 3apos or 3apos to 5apos) of the probe sequence |
| Used in Microarray | Human Intestinal Bacteria Chip |
Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.
