Calculating ...

Press ESC to cancel.

Probe/primer details

Name DFMI227b
Full name
(Alm et al. 1996)
S-*-DfmI-0227-b-A-18
Accession no. pB-512
Taxonomy Desulfotomaculum; Peptococcaceae; Clostridiales; Clostridia; Firmicutes; Bacteria
Specificity Desulfotomaculum aeronauticum, Dfm. nigrificans, Dfm. reducens, Dfm. ruminis, Dfm. sapomandens, Dfm. halophilum
Category sulfate-reducing microbes
Target rRNA 16S rRNA
Modified version(s) DFM228
DFMI227a
Position 227-244
Sequence 5'- ATG GGA CGC GGA TCC ATC -3'
G+C content [%] 61
Length [nt] 18
Check specificity/coverage
Hybridization efficiency
References

Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment. Loy A, Lehner A, Lee N, Adamczyk J, Meier H, Ernst J, Schleifer KH, Wagner M. Applied and environmental microbiology. 2002. Pubmed

Remarks Use probes DFMI227a and DFMI227b in equimolar mixture to detect all Desulfotomaculum spp. of cluster I
Used in Microarray SRP-PhyloChip

Print

Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.