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Probe/primer details

DSB706 Tested for in situ hybridization.
Full name
(Alm et al. 1996)
S-*-Dsb-0706-a-A-18
Accession no. pB-436
Taxonomy Desulfobulbaceae; Desulfobacterales; Deltaproteobacteria; delta/epsilon subdivisions; Proteobacteria; Bacteria
Specificity Most Desulfobulbaceae and Thermodesulforhabdus
Category sulfate-reducing microbes
Target rRNA 16S rRNA
Position 706-723
Sequence 5'- ACC GGT ATT CCT CCC GAT -3'
G+C content [%] 56
Length [nt] 18
Check specificity/coverage
Formamide [%] 20-55 (45)
Hybridization efficiency
References

Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment. Loy A, Lehner A, Lee N, Adamczyk J, Meier H, Ernst J, Schleifer KH, Wagner M. Applied and environmental microbiology. 2002. Pubmed

Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization. Lücker S, Steger D, Kjeldsen KU, MacGregor BJ, Wagner M, Loy A. Journal of microbiological methods. 2007. Pubmed

Used in Microarray SRP-PhyloChip

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Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.