Probe/primer details
| Lbd | Tested for in situ hybridization. |
| Accession no. | pB-3937 |
| Taxonomy | Lactobacillus delbrueckii; Lactobacillus; Lactobacillaceae; Lactobacillales; Bacilli; Firmicutes; Bacteria |
| Specificity | Lactobacillus delbrueckii subsp. |
| Category | animal and human associated microbiota intestinal microbiota |
| Target rRNA | 23S rRNA |
| Sequence | 5'- AAG GAT AGC ATG TCT GCA -3' |
| G+C content [%] | 44 |
| Check specificity/coverage | 
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| Hybridization efficiency |  |
| References | Identification of lactobacillus delbrueckii and subspecies by hybridization probes and PCR. Lick S, Brockmann E, Heller KJ. Systematic and applied microbiology. 2000. Pubmed Hertel et al (1993) Differentiation of lactobacilli occurring in fermented milk products by using oligonucleotide probes and electrophoretic protein profiles. In Systemiatic and Applied Microbiology, Volume 16, Issue 3, November 1993, Pages 463–467. |
| Remarks | Hybridization temperature 40° C, washing temperature 50° C (according to Lick et al., 2000) |
Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.
