Calculating ...

Press ESC to cancel.

Probe/primer details

UNIV1390 Tested for in situ hybridization.
Full name
(Alm et al. 1996)
S-*-Univ-1390-a-A-18
Accession no. pB-327
Taxonomy cellular organisms
Specificity all Organisms
Category higher taxonomic levels
Target rRNA 16S rRNA
Modified version(s) UNIV1389a
UNIV1389b
UNIV1389c
3
Position 1390-1407
Sequence 5'- GAC GGG CGG TGT GTA CAA -3'
G+C content [%] 61
Length [nt] 18
Check specificity/coverage
Hybridization efficiency
References

Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies. Zheng D, Alm EW, Stahl DA, Raskin L. Applied and environmental microbiology. 1996. Pubmed

Used in Microarray Nitrifier-Microarray
Freshwater Sediment-Microarray

Print

Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.